http://tede2.uefs.br:8080/handle/tede/2 2026-09-11T01:39:34Z 2026-09-11T01:39:34Z Barbosa, Deyse Brito http://tede2.uefs.br:8080/handle/tede/2175 2026-09-10T16:59:36Z 2025-06-30T00:00:00Z

Título: Planejamento de inibidores triplos frente à acetilcolinesterase, butirilcolinesterase e beta-secretase 1 Autor: Barbosa, Deyse Brito Primeiro orientador: Leite, Franco Henrique Andrade Abstract: Alzheimer’s disease (AD) is a multifactorial neurodegenerative chronical disorder that primarily affects the elderly. Despite impacts in patients’ life, current pharmacological treatments show limited efficacy, highlighting the urgent need for new therapeutic options. For this reason, multitarget compounds capable of simultaneously inhibiting key enzymes such as acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and beta-secretase 1 (BACE-1) have emerged as promising candidates and, in regard of multitarget identification, drug repurposing has proven to be an effective strategy. Previous virtual screening identified silodosin, an FDA-approved drug for treat urological conditions, as a potential triple inhibitor for AD treatment (QFIT=31.67; ScoreAChE=-8.0kcal/mol; ScoreBuChE=-8.1kcal/mol; ScoreBACE-1=-43.55). This study evaluated silodosin and its derivatives using in vitro assays and molecular dynamics (MD) simulations. Silodosin was shown to be safe at concentrations below 10μM, with low AChE inhibition (IC₅₀>500μM) but satisfactory activity against BuChE and BACE-1. MD simulations confirmed these findings, revealing greater complex stability with BuChE and BACE-1. Rational design of new derivatives led to improved AChE affinity, with structural modifications such as simplification of the linker chain and introduction of smaller substituents. Derivative S23 demonstrated a promising binding profile (ScoreAChE =-9.8kcal/mol; ScoreBuChE=-7.7kcal/mol; ScoreBACE-1=- 39.74) and was selected for synthesis and further evaluation. These findings support the viability of the multi-target approach and contribute to the development of new therapeutic candidates for AD. Instituição: Universidade Estadual de Feira de Santana Tipo do documento: Tese

2025-06-30T00:00:00Z Carneiro, Helanya Santana http://tede2.uefs.br:8080/handle/tede/2174 2026-09-10T04:00:07Z 2024-08-22T00:00:00Z

Título: Cancioneiro do sertão: o vocabulário dos cordéis de Franklin Maxado Autor: Carneiro, Helanya Santana Primeiro orientador: Barreiros, Liliane Lemos Santana Abstract: This thesis produced 854 entries on the vocabulary of the backlands of Franklin Maxado, a writer from Feira de Santana, Bahia, after answering two research questions: What contributions can the systematization of the backlands vocabulary in Franklin Maxado's works offer to the documentation of the author's collection? And to what extent does the study of Franklin Maxado's lexicon reflect the culture and customs of the backlands man? The corpus was delimited from three collections of backlands, dating from the mid-1970s to 2022. There are 204 backlands belonging to the researcher, 23 were constituted by the library of the Casa Museu do Sertão and 09 erotic ones also from the same place, but written under the pseudonym K. Gay Nawara, totaling 236 texts. The entries of the entries were selected with the help of the AntConc program, which listed the lexias and their frequency in the author's texts. The research is supported by the parameters of Lexicology, Lexicography and Metalexicography (Biderman, 1978; 2001; Rey-Debove, 1984; Vilela, 1995; Barbosa, 2001; Porto Dapena, 2002; Isquerdo, 2006, Zavaglia, 2012, Barreiros 2017 and others) for the elaboration of lexicographical works and brings a discussion on popular literature, cordel literature, folklore, as well as the nomenclatures cordel and folhalho, based on studies on culture, orality and cordel in Brazil and Portugal, according to Melo (2020), Abreu (1999), Rocha (1998), Aragão (2004), Mendes (2010), Lemaire (2010), Matos (2010), Santos (2010), Maxado (2012), Zumthor (2014) and others. Franklin Maxado's Vocabulary is a significant source of historical, political and sociocultural information about the backlands, based on regional language, which highlights a peculiar use of the lexicon. In this way, this research contributes to the understanding, dissemination and preservation of the customs and cultural values of the backlands, expressed through language. Instituição: Universidade Estadual de Feira de Santana Tipo do documento: Tese

2024-08-22T00:00:00Z Sousa, Amanda Bahiano Passos http://tede2.uefs.br:8080/handle/tede/2173 2026-09-09T04:00:10Z 2026-03-13T00:00:00Z

Título: Resistência genética de Musa spp. A Radopholus similis: fundamentos metodológicos, caracterização fenotípica e molecular Autor: Sousa, Amanda Bahiano Passos Primeiro orientador: Amorim, Edson Perito Abstract: Banana is one of the most important fruit crops worldwide and plays a fundamental role in global food security. Among the main constraints to banana production are plantparasitic nematodes, particularly Radopholus similis, one of the most destructive species associated with Musa spp. In this context, genetic resistance represents a sustainable and durable strategy for managing this pathogen. This study first conducted a systematic review of the literature published between 2011 and 2024 to identify sources of genetic resistance to nematodes in Musa spp. Following PRISMA guidelines, a standardized search strategy and predefined inclusion and exclusion criteria were applied across multiple academic databases, resulting in the selection of 69 studies. The review confirmed R. similis as the most extensively investigated nematode species worldwide and highlighted the existence of promising resistance sources within Musa germplasm. The resistance response of twenty banana genotypes from different regions of Brazil was also evaluated based on symptom severity and nematode reproduction factor. A wide variation in response was observed among genotypes of the Prata subgroup (AAB), whereas Cavendish subgroup (AAA) genotypes were predominantly susceptible. Additionally, the infection process of R. similis in roots of improved diploid banana genotypes, previously identified as contrasting in their response to the nematode, was investigated at histochemical and molecular levels. Gene expression analyses revealed hormonal and structural events associated with root resistance. Finally, a diagrammatic scale was developed and validated to improve the assessment of host response to R. similis infection, based on the extent of rhizome necrosis in banana seedlings. Rhizome area and symptom severity were quantified using ImageJ software, and scale validation involved ten inexperienced evaluators who estimated the severity of one hundred rhizomes with and without the use of the scale. The application of the scale significantly increased interrater agreement, improving R² and Lin’s concordance correlation coefficient (CCC) values and reducing systematic bias in severity estimates. Taken together, this study integrates systematic evidence, methodological advances, and experimental validation, providing novel contributions and practical tools to support Musa breeding programs focused on resistance to R. similis. Instituição: Universidade Estadual de Feira de Santana Tipo do documento: Tese

2026-03-13T00:00:00Z Oliveira, Yuri Andrade de http://tede2.uefs.br:8080/handle/tede/2172 2026-09-09T04:00:12Z 2026-03-26T00:00:00Z

Título: Atividade antimicrobiana de nanoemulsões de óleos essenciais frente a patógenos associados a candidose e a periodontite Autor: Oliveira, Yuri Andrade de Primeiro orientador: Trindade, Soraya Castro Abstract: The research is based on the search for therapeutic alternatives for the management of oral candidiasis and periodontitis, conditions frequently associated with dysbiosis and the formation of resilient biofilms. Although essential oils possess relevant antimicrobial properties, their clinical application is limited by high volatility, low solubility, and chemical instability. In this scenario, the use of nanoemulsions emerges as a biotechnological strategy to protect these compounds and increase their bioavailability. Therefore, the objective of the study was to develop, characterize, and evaluate the antimicrobial efficacy of nanoemulsions containing essential oils of Cymbopogon flexuosus, Ocimum basilicum, Lavandula dentata, Lavandula angustifolia, and Eugenol against opportunistic pathogens, correlating their performance with physicochemical properties and cytotoxicity profiles. For this purpose, the preparation of the colloidal systems was performed using high and lowenergy methods, followed by characterization regarding droplet size, Zeta potential, and stability. Biological activity was measured through the determination of Minimum Inhibitory Concentration (MIC) and Minimum Microbicidal Concentration (MMC) against reference strains (Candida albicans ATCC 10231 and Nakaseomyces glabrata ATCC 15126; Fusobacterium nucleatum subsp. nucleatum ATCC 25586, Veillonella parvula ATCC 10790, Porphyromonas gingivalis ATCC 33277, Prevotella melaninogenica ATCC 25845, and Eikenella corrodens ATCC 23864) and clinical isolates from patients with candidiasis and periodontitis (subgingival biofilm) using silver nanoparticles as a comparative reference, in addition to cell viability assays in human lineages. With the development of nanoemulsions that enhanced the action of essential oils against oral pathogens as a distinguishing factor. The results demonstrated that the obtained nanoemulsions presented robust stability and nanometric dimensions below 130 nm, which favored interaction with microbial membranes. It was observed that nanostructuring significantly enhanced the action of the corresponding free actives; for instance, the nanoemulsion containing Eugenol reduced the dose required to inhibit Fusobacterium nucleatum by up to eight times compared to the free oil. Additionally, the formulation containing Ocimum basilicum enabled potent antifungal action, while cytotoxicity assays indicated that the lowenergy method better preserved cell viability. It is concluded that nanoemulsions containing essential oils represent an effective and safe alternative therapy, overcoming the limitations of free-state compounds and offering a promising alternative for controlling complex oral infections and combating microbial resistance. Instituição: Universidade Estadual de Feira de Santana Tipo do documento: Tese

2026-03-26T00:00:00Z