Morfogênese e conservação in vitro de Caesalpinia pyramidalis Tul.

Abstract

The catingueira (Caesalpinia pyramidalis Tul.) is an endemic species of the Caatinga, which has the potential timber, fodder and medicinal. The plant tissue culture has proved effective in multiplication and conservation of woody species with potential explored. Thus, this study investigated the effects of the types and concentrations of plant growth regulators on in vitro multiplication of C. pyramidalis and develop a protocol for in vitro conservation of the same. For multiplication was carried out three experiments in which we assessed the effects of different concentrations of plant growth regulators (BAP, KIN, TDZ or NAA) on different explants (hypocotyls, cotyledon segments, cotyledons, and epicotyl nodal segments). For the conservation in vitro experiments there were two: firstly tested the effect of different concentrations of sucrose (87.64, 131.46, 175.28 and 219.10mM) supplemented with different concentrations (0.0 and 87.64mM) of sorbitol or mannitol, and second, to evaluate the use of different concentrations (0.0, 1.5, 3.0, 4.5 and 6.0µM) the growth retardant paclobutrazol (PBZ). The culture medium used for all experiments was the WPM. The highest rate of regeneration, shoot number, leaf number, shoot length and dry weight of shoots were obtained from the nodal segments were inoculated into plant growth regulator free medium. The use of mannitol and sorbitol were not efficient for in vitro conservation catingueira, being suitable for this purpose, use of concentration of 219.10mM sucrose and 6μM PBZ until 240 days.