Avaliação do potencial antiinflamatório das células mononucleares de medula óssea em camundongos com epilepsia induzida por cloreto de pilocarpina

Abstract

The temporal lobe epilepsy (TLE) is the most frequent form of epilepsy, standing for approximately 30% of cases in adults and the complex partial seizures is its typical manifestation. This type of involvement is often refractory to drug treatment and the main symptoms are generated predominantly by the involvement of the medial temporal lobe structures. Recently, some genes related to inflammatory processes, such as interleukin-1 beta (IL-1), have been implicated in TLE in humans and in animal models. Stem cells represent a hope for epilepsy treatment, as they have great capacity for proliferation, differentiation, tissue regeneration and immunomodulation. Our main study goals were to assess the potential of bone marrow mononuclear cells (BMMC) on the control of inflammatory responses and their effects on the cellular model of neuronal loss in pilocarpine-induced epilepsy in mice. After being induced epilepsy model (status epilepticus - SE), animals were transplanted with live and lysed BMMC obtained from C57BL/6 GFP transgenic mice. Animals were euthanized at 12 h, 24 h and 7 days after transplantation. We evaluated the pro-inflammatory and anti-inflammatory expression profile on serum samples and brain tissue. Our results demonstrated that both cell therapies employed were able to reduce the pro-inflammatory response as well as stimulate an antiinflammatory response. Based on neuronal quantification we noted that transplanted animals showed a smaller loss of hippocampal neurons in regions CA1 and CA3. We believe the results obtained in this study may contribute to the understanding of a mechanism of action of these cells in an experimental model of epilepsy.