Avaliação da Myracrodruon urundeuva Fr. Allemão sob diferentes formas de cultivo: crescimento, atividade antimicrobiana e compostos fenólicos

Abstract

Techniques in vitro and ex vitro of culture have been considered promising approaches front of the increasing interest in the plants that can be used as medicine. Aiming to evaluate the growth, the antibacterial activity and the concentration of phenolic compounds from Myracrodruon urundeuva under different forms of cultivation, were tested two culture medium for in vitro establishment and different concentrations of 2,4-D (2,4- dichlorophenoxyacetic acid) to callus induction, also of submit the seed to priming with -1,0 MPa of PEG 6000 (polyethyleneglycol) under constant aeration and for different periods and conditions pre-germination, and then sown in a greenhouse. These samples was determined the antimicrobial activity of the leaves and the concentration of phenolic compounds. The results showed that the WPM (Wood Plant Medium) was the best medium for the establishment and initial growth of seedlings in vitro, the concentration of 1,25mg.L-1 of 2,4-D promoted more callus formation using leaf segment; that the priming was not effective in improving seed germination, but accelerated and standardized on the plants in a greenhouse, and that the leaves of plants grown ex vitro presented antimicrobial activity against the bacteria tested, without activity for the candid, which may be related to the concentration of phenolic compounds was higher in plants grown ex vitro and from the priming. Therefore, it was inferred that the environment and how to grow seeds influenced the production of phenolic compounds and the antimicrobial activity of leaves of M. urundeuva.